| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.074340 |
| Chromosome: | chromosome 11 |
| Location: | 2173352 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre11.g469750 | (1 of 11) PF07173 - Protein of unknown function (DUF1399) (DUF1399) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCAAAACCGGTCTATCCGCCGCCGCGCCCG |
| Internal bar code: | GACGGTGTTTCATTCACTGTCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 43 |
| LEAP-Seq percent confirming: | 57.764 |
| LEAP-Seq n confirming: | 93 |
| LEAP-Seq n nonconfirming: | 68 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTGTGCAAACCTTTCGTGTG |
| Suggested primer 2: | CTGTGGTCGCTTTTTGGTTT |