| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.074346 |
| Chromosome: | chromosome 9 |
| Location: | 4581725 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g396326 | (1 of 1) PF02213 - GYF domain (GYF) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AAGTGTTGGGATCACGTTGGCGCGACGTCA |
| Internal bar code: | TGAACAATTTCAAAAGCACGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 331 |
| LEAP-Seq percent confirming: | 98.517 |
| LEAP-Seq n confirming: | 4916 |
| LEAP-Seq n nonconfirming: | 74 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATGTGTATGTGCGCGTGTTT |
| Suggested primer 2: | GTACACGCCCGAGTCAAAGT |