| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.074346 |
| Chromosome: | chromosome 14 |
| Location: | 3257599 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g629429 | (1 of 41) PTHR32523//PTHR32523:SF8 - FAMILY NOT NAMED // PHYTOL KINASE 1, CHLOROPLASTIC | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCCCATGCTGTGTTGGTGCGGGCGGGCGCG |
| Internal bar code: | TCAGGCTAAGGTGACGCACGAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 381 |
| LEAP-Seq percent confirming: | 99.3406 |
| LEAP-Seq n confirming: | 12052 |
| LEAP-Seq n nonconfirming: | 80 |
| LEAP-Seq n unique pos: | 26 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCAAGCTGCTGCATTGATAG |
| Suggested primer 2: | CAGGCTCTTTATGACGGCTC |