Insertion junction: LMJ.RY0402.074349_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.074349
Chromosome:chromosome 3
Location:6747309
Confidence (%):58
Locus systematic id Locus common name Defline Feature
Cre03.g198050 (1 of 2) IPR002913//IPR023393 - START domain // START-like domain CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CAAGGGCCTGTTCATCTACAAGTCGAAGAC
Internal bar code:GTCGACCATGAGCCAACAGCAC

Confirmation - LEAP-Seq

LEAP-Seq distance:15
LEAP-Seq percent confirming:99.5215
LEAP-Seq n confirming:624
LEAP-Seq n nonconfirming:3
LEAP-Seq n unique pos:2

Suggested primers for confirmation by PCR

Suggested primer 1:CACATTAACTCGAAAGCGCA
Suggested primer 2:GTAGCCTGAGACGAAGTCCG