| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.074356 |
| Chromosome: | chromosome 12 |
| Location: | 2529795 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g505900 | TIM50 | (1 of 1) K17496 - mitochondrial import inner membrane translocase subunit TIM50 (TIM50) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGTCATGTTACAGGGCGAACCTTTCCATTC |
| Internal bar code: | GACTTAACCCCACGCGCTAGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 182 |
| LEAP-Seq percent confirming: | 99.0847 |
| LEAP-Seq n confirming: | 433 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTTCTGCCATCATGCTCTTG |
| Suggested primer 2: | AAAGCCTACTCCGGGTGATT |