| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.074392 |
| Chromosome: | chromosome 1 |
| Location: | 5062934 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g035000 | (1 of 1) K14791 - periodic tryptophan protein 1 (PWP1) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCAGCCGTCCCCCTCCGCCGGCACGTCCCC |
| Internal bar code: | GAGTCCTTCGTATCCCTGCCTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 100 |
| LEAP-Seq percent confirming: | 85.0 |
| LEAP-Seq n confirming: | 119 |
| LEAP-Seq n nonconfirming: | 21 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCTGACATGGACTCTGACGA |
| Suggested primer 2: | TCAGCGCACTATGAGTTTGG |