Insertion junction: LMJ.RY0402.074392_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.074392
Chromosome:chromosome 1
Location:5063239
Confidence (%):58
Locus systematic id Locus common name Defline Feature
Cre01.g035000 (1 of 1) K14791 - periodic tryptophan protein 1 (PWP1) CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GGGCTGGGTTAGGTGGGGTGGGGGTGGAGG
Internal bar code:CCTCGTCATGTGCTAAACTCGC

Confirmation - LEAP-Seq

LEAP-Seq distance:12
LEAP-Seq percent confirming:43.8181
LEAP-Seq n confirming:925
LEAP-Seq n nonconfirming:1186
LEAP-Seq n unique pos:2

Suggested primers for confirmation by PCR

Suggested primer 1:GCTGACATGGACTCTGACGA
Suggested primer 2:CAGGTAAGGAAGGGGAGAGG