| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.074392 |
| Chromosome: | chromosome 1 |
| Location: | 5063560 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g035000 | (1 of 1) K14791 - periodic tryptophan protein 1 (PWP1) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TACCTGCAGCTCGGCGACGACGACTCGGAA |
| Internal bar code: | GAGGACTGCAGAAGGGGGGCCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 535 |
| LEAP-Seq percent confirming: | 93.2906 |
| LEAP-Seq n confirming: | 4658 |
| LEAP-Seq n nonconfirming: | 335 |
| LEAP-Seq n unique pos: | 18 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCGCTACTAGCCTTGTTTGG |
| Suggested primer 2: | CACACCACACCATTAGCCAG |