| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.074405 |
| Chromosome: | chromosome 12 |
| Location: | 4311017 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g519950 | FAP42,GUK3,C1b-350 | Flagellar Associated Protein 42; (1 of 1) PTHR23359//PTHR23359:SF92 - NUCLEOTIDE KINASE // SUBFAMILY NOT NAMED | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTGGGGCCGGTGATGACGACGGGCTTGAGG |
| Internal bar code: | TGGCAGTGACACCCGCCAAGGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 200 |
| LEAP-Seq percent confirming: | 98.8764 |
| LEAP-Seq n confirming: | 440 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGCGACTACTTCTTTTGCGA |
| Suggested primer 2: | GTGACAGGGATTTGAGTGGG |