| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.074432 |
| Chromosome: | chromosome_2 |
| Location: | 2228435 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre02.g089950 | IFT20 | Intraflagellar transport protein 20 | antisense | intron |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | TGACGCGAGTGTCAGGCTGAGCGTGCCGGG |
| Internal bar code: | GCGTACAGGCGATCGGGGCAAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 375 |
| LEAP-Seq percent confirming: | 80.5283 |
| LEAP-Seq n confirming: | 4024 |
| LEAP-Seq n nonconfirming: | 973 |
| LEAP-Seq n unique pos: | 19 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TAGCGTTAGGCGAGTCGTTT |
| Suggested primer 2: | ATTAGTCTTGGCGGGTGTTG |