| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.074436 |
| Chromosome: | chromosome 6 |
| Location: | 1781772 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g262350 | (1 of 1) IPR003722 - Cobalamin (vitamin B12) biosynthesis CobH/CbiC, precorrin-8X methylmutase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGTAGGGCTTCATCCCAGCGACACCGCGGC |
| Internal bar code: | ACAACGCCCAGAGCCTATGTGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 101 |
| LEAP-Seq percent confirming: | 67.1815 |
| LEAP-Seq n confirming: | 348 |
| LEAP-Seq n nonconfirming: | 170 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCGCGAAACATGTCTATCAA |
| Suggested primer 2: | CGAAGACAATAAGCGAAGCC |