| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.074436 |
| Chromosome: | chromosome 17 |
| Location: | 3347961 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g723500 | (1 of 1) 3.6.3.30 - Fe(3+)-transporting ATPase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGTTCGACGTGGCGCGTGTGGCCACGTACG |
| Internal bar code: | TTCGCGCGATCTTACATCCGTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 310 |
| LEAP-Seq percent confirming: | 99.9322 |
| LEAP-Seq n confirming: | 1474 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCCAGCATGAGCGTTAGTTG |
| Suggested primer 2: | GTTAGATGCCCAGAAAACGC |