Insertion junction: LMJ.RY0402.074454_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.074454
Chromosome:chromosome 6
Location:6327840
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre06.g291850 (1 of 6) PTHR24114:SF2 - LEUCINE-RICH REPEAT-CONTAINING PROTEIN 74 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CGCGTGCCTCGCCTAAAAGACGCCGCCGCC
Internal bar code:GTGTGCTGTCTGGCGAAGAGGT

Confirmation - LEAP-Seq

LEAP-Seq distance:372
LEAP-Seq percent confirming:81.9467
LEAP-Seq n confirming:2029
LEAP-Seq n nonconfirming:447
LEAP-Seq n unique pos:12

Suggested primers for confirmation by PCR

Suggested primer 1:GTGTACACCACCAGCGATTG
Suggested primer 2:TGATCCTGCAGCACTACGAC