| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.074454 |
| Chromosome: | chromosome 6 |
| Location: | 6328215 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g291850 | (1 of 6) PTHR24114:SF2 - LEUCINE-RICH REPEAT-CONTAINING PROTEIN 74 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TAAACCCCAGCCAACCCGACTCACGCGCGC |
| Internal bar code: | CGGATAAATTGTTATATGTTGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 271 |
| LEAP-Seq percent confirming: | 97.54 |
| LEAP-Seq n confirming: | 1705 |
| LEAP-Seq n nonconfirming: | 43 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAAGGGAAGAGAGGGAATGG |
| Suggested primer 2: | GTTGTGGAATGTGGCTGATG |