| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.074463 |
| Chromosome: | chromosome 1 |
| Location: | 2088753 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g011200 | (1 of 1) PF04658 - TAFII55 protein conserved region (TAFII55_N) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GAGCTCAAGGGTGGGTCGGCGCAGTAGGGT |
| Internal bar code: | TCGACGTTGGAAGAAGCGAGGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 155 |
| LEAP-Seq percent confirming: | 70.0 |
| LEAP-Seq n confirming: | 203 |
| LEAP-Seq n nonconfirming: | 87 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGGAGCTGTTTGTTTTGCGT |
| Suggested primer 2: | GCAGAGGGTGTGTGGTAGGT |