Insertion cassette: | CIB1 |
Side of cassette: | 5' |
Strand: | + |
Strain: | LMJ.RY0402.074469 |
Chromosome: | chromosome_12 |
Location: | 6129034 |
Confidence (%): | 73 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre12.g536000 | ALA2 | ATPase, aminophospholipid transporter | antisense | 3'UTR |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | CCGTATTAGGACGGTGCCCGGACTACCAAG |
Internal bar code: | TGGAGCCCCATTTTGTCTGTCG |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 535 |
LEAP-Seq percent confirming: | 99.6392 |
LEAP-Seq n confirming: | 10217 |
LEAP-Seq n nonconfirming: | 37 |
LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | TTGGTCACGTGGACCTTACA |
Suggested primer 2: | CATTGTGTTTGCTGTCTGGG |