| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.074475 |
| Chromosome: | chromosome 16 |
| Location: | 6158973 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g675450 | SDR25 | Short-chain dehydrogenase/reductase; (1 of 1) PTHR24322//PTHR24322:SF383 - FAMILY NOT NAMED // L-XYLULOSE REDUCTASE-RELATED | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGCCGTACTGTCAATTTGTGGGGCGCGATA |
| Internal bar code: | GCTTCCACGTTGTGCGAACGGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 313 |
| LEAP-Seq percent confirming: | 56.0696 |
| LEAP-Seq n confirming: | 5284 |
| LEAP-Seq n nonconfirming: | 4140 |
| LEAP-Seq n unique pos: | 28 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGCGCCCTTCACTACTTTCA |
| Suggested primer 2: | TTGCTGTACGACAGGTTTGC |