Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | - |
Strain: | LMJ.RY0402.074477 |
Chromosome: | chromosome 3 |
Location: | 5315157 |
Confidence (%): | 58 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre03.g183750 | OPR16 | OctotricoPeptide Repeat protein 16 | 5'UTR |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGCTTCCGGCGTTGCTTTGCGGGGCGAACA |
Internal bar code: | ACGGAGCCAAGCGCTAAAGGCG |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 432 |
LEAP-Seq percent confirming: | 90.9852 |
LEAP-Seq n confirming: | 1413 |
LEAP-Seq n nonconfirming: | 140 |
LEAP-Seq n unique pos: | 16 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | GACCTACCCCACACGACACT |
Suggested primer 2: | AGTCACCTGCGCGTTAAACT |