| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.074499 |
| Chromosome: | chromosome 1 |
| Location: | 6812564 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g049117 | 5'UTR_intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGTATCAACGTACGCCAACTCAGTTGGTTC |
| Internal bar code: | CGTCCGATGGGAACATACGGAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 977 |
| LEAP-Seq percent confirming: | 98.5547 |
| LEAP-Seq n confirming: | 8183 |
| LEAP-Seq n nonconfirming: | 120 |
| LEAP-Seq n unique pos: | 38 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGCTAGCTAGTGGATGCCTG |
| Suggested primer 2: | AACCGACCTCCACAACAAAG |