| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.074504 |
| Chromosome: | chromosome 17 |
| Location: | 4015191 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g728976 | (1 of 1) IPR000104//IPR001254//IPR009003 - Antifreeze protein, type I // Serine proteases, trypsin domain // Peptidase S1, PA clan | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AATACACCCCGCCACCACACACTGCACGTG |
| Internal bar code: | GATGCCTTTACGCGTTGGGTGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 678 |
| LEAP-Seq percent confirming: | 88.1901 |
| LEAP-Seq n confirming: | 4824 |
| LEAP-Seq n nonconfirming: | 646 |
| LEAP-Seq n unique pos: | 23 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAATGCCACTGCTGCTGTTA |
| Suggested primer 2: | TGGGGATTGTGTTTTTGGAT |