| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.074515 |
| Chromosome: | chromosome 16 |
| Location: | 1419046 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g652300 | CMA2,TTL13 | Chlamydomonas-specific membrane protein family A | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTGGGAAGCCCGGTCCAATGCTAAGCCTGC |
| Internal bar code: | CACGGCGACCCGCCCCAGGGCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 122 |
| LEAP-Seq percent confirming: | 67.8477 |
| LEAP-Seq n confirming: | 4098 |
| LEAP-Seq n nonconfirming: | 1942 |
| LEAP-Seq n unique pos: | 9 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATCTATGCAATCCCTGGCAC |
| Suggested primer 2: | TGTTGCTTCCCCACTTTTTC |