| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.074518 |
| Chromosome: | chromosome 1 |
| Location: | 7250035 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g052300 | E2F1 | (1 of 1) K06620 - transcription factor E2F3 (E2F3); Transcription factor, E2F and DP-related | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGTCCTGGTCGTCGGGGTCCGCTCCCCGGT |
| Internal bar code: | CAGTTATCCGTGGGGCGCAGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 128 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 24 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCGAGCTGTACCATCACTCA |
| Suggested primer 2: | CCTGAACACACATGCCAAAC |