| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.074523 |
| Chromosome: | chromosome 16 |
| Location: | 2512843 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g661000 | THB7 | (1 of 2) PTHR10681//PTHR10681:SF17 - THIOREDOXIN PEROXIDASE // PEROXIREDOXIN-6; Truncated hemoglobin | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TAATCTACCCACGCCGGGAGTGACCCATGC |
| Internal bar code: | GCTGGCTAGACTCCCGTGGGGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 17 |
| LEAP-Seq percent confirming: | 1.70213 |
| LEAP-Seq n confirming: | 8 |
| LEAP-Seq n nonconfirming: | 462 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACACCGCCTATGCGAGATAC |
| Suggested primer 2: | AAGCGAAGTGGGACTTCTGA |