| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.074540 |
| Chromosome: | scaffold 19 |
| Location: | 34067 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre19.g750397 | (1 of 1) 3.2.1.17 - Lysozyme / Muramidase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGCGGCAGCACTGCACCATGCCAGAACGGC |
| Internal bar code: | GCAGTCAGTGACGCATAGCGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 223 |
| LEAP-Seq percent confirming: | 78.941 |
| LEAP-Seq n confirming: | 3265 |
| LEAP-Seq n nonconfirming: | 871 |
| LEAP-Seq n unique pos: | 9 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGATAGCGGCAGCTTACTTG |
| Suggested primer 2: | ACACACACACACACACACCG |