| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.074544 |
| Chromosome: | chromosome 7 |
| Location: | 5852573 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g353600 | MMP7 | Metalloproteinase of VMP family; (1 of 40) 3.4.24.38 - Gametolysin / Lysin | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CATATACCAGACTGCCAGGCATACGCAATG |
| Internal bar code: | CGTAATAACTATCGTCGTCTCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 809 |
| LEAP-Seq percent confirming: | 99.8774 |
| LEAP-Seq n confirming: | 7330 |
| LEAP-Seq n nonconfirming: | 9 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTGCTGCATCTGTATCGCAT |
| Suggested primer 2: | ATGCCGTGAAATTTGGTAGC |