| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.074551 |
| Chromosome: | chromosome 3 |
| Location: | 3119527 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g164900 | (1 of 1) PTHR24361//PTHR24361:SF159 - MITOGEN-ACTIVATED KINASE KINASE KINASE // SERINE/THREONINE-PROTEIN KINASE OSR1 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GACAGCGCCAGGCGTGGCGCGGGCGCGGAT |
| Internal bar code: | ATAGATTAAGACGCCGGCATGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 45 |
| LEAP-Seq percent confirming: | 78.1095 |
| LEAP-Seq n confirming: | 1413 |
| LEAP-Seq n nonconfirming: | 396 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCTCAGCACTCCAAATGACA |
| Suggested primer 2: | ATGGTCTGCTCCATGACCTC |