| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.074570 |
| Chromosome: | chromosome 7 |
| Location: | 1712580 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g325710 | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTCTCCGGGACTTGAGCCGGGGACGCGACC |
| Internal bar code: | TAAACTATGCCGGCCGTAACG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 145 |
| LEAP-Seq percent confirming: | 95.3737 |
| LEAP-Seq n confirming: | 268 |
| LEAP-Seq n nonconfirming: | 13 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAGCTTACCTCCTGCCAAAG |
| Suggested primer 2: | CAGCAGCAGCAGCAGAGC |