| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.074582 |
| Chromosome: | chromosome 17 |
| Location: | 444446 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g699000 | PAT1 | Phosphate acetyltransferase; (1 of 2) 2.3.1.8 - Phosphate acetyltransferase / Phosphotransacetylase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTGTGCGGCACAGAGAGCGTGTGTGTACGG |
| Internal bar code: | GCTTCAAATATGGTTTGCGCGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 595 |
| LEAP-Seq percent confirming: | 74.9022 |
| LEAP-Seq n confirming: | 3829 |
| LEAP-Seq n nonconfirming: | 1283 |
| LEAP-Seq n unique pos: | 17 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCCAGTAGCGACCAAGAGTC |
| Suggested primer 2: | CTACCCTCCCACTCTCCCTC |