| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.074589 |
| Chromosome: | chromosome 6 |
| Location: | 7725716 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g302000 | MFP31,MFP1 | Major facilitator superfamily transporter; (1 of 2) PF05977 - Transmembrane secretion effector (MFS_3) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCAGAAGAAGGCGGGCAGTGCATGTCATGA |
| Internal bar code: | GCGAGCACCGGTTCGCACGTAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 705 |
| LEAP-Seq percent confirming: | 99.6341 |
| LEAP-Seq n confirming: | 817 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCTCACTCCTCGTCAAGTCC |
| Suggested primer 2: | CGTGTTGTACCCGTATGCAG |