| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.074612 |
| Chromosome: | chromosome 6 |
| Location: | 6143819 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g290200 | CYG39 | (1 of 70) 4.6.1.2 - Guanylate cyclase / Guanylyl cyclase; Adenylate/guanylate cyclase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTTAAGAAGGGAAGGCGTAGCCAGGGGGGG |
| Internal bar code: | CGCAAAGTGGTGATTCCTTCGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1 |
| LEAP-Seq percent confirming: | 60.0 |
| LEAP-Seq n confirming: | 96 |
| LEAP-Seq n nonconfirming: | 64 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAGACTCTCTATCGCCCGAC |
| Suggested primer 2: | TTGCAGGCGTTAGTGTTCTG |