| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.074636 |
| Chromosome: | chromosome 17 |
| Location: | 4566929 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g732850 | SCO1 | (1 of 1) K07152 - protein SCO1/2 (SCO1_2); Cytochrome c oxidase assembly factor, SCO1/SenC | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGGAAAGGGGCCGGAAGTTGCATTGCCACG |
| Internal bar code: | GGTTTTCGAGGGAACGATCCCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 190 |
| LEAP-Seq percent confirming: | 99.0566 |
| LEAP-Seq n confirming: | 210 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGTCTCCTCCAGGCATTACA |
| Suggested primer 2: | AACACGGTGGAGAAGTGGAC |