| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.074640 |
| Chromosome: | chromosome 6 |
| Location: | 4149357 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g278227 | (1 of 4) 2.3.1.12 - Dihydrolipoyllysine-residue acetyltransferase / Transacetylase X | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTCACGCCATGAACGTGATGCGCCACAACC |
| Internal bar code: | GAAGGTATGGGCCTCGGCCTAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 500 |
| LEAP-Seq percent confirming: | 99.7436 |
| LEAP-Seq n confirming: | 4668 |
| LEAP-Seq n nonconfirming: | 12 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TATACCGGTAGGGCTGTTGC |
| Suggested primer 2: | ATGAGTAGAGCCCTCGCAAA |