| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.074648 |
| Chromosome: | chromosome 3 |
| Location: | 355644 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g144787 | TRL12 | (1 of 11) 1.8.1.8 - Protein-disulfide reductase / Protein disulfide reductase; Thioredoxin-like protein | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGGATGCAGAGCCGCCGGAGAGTCTAGGGT |
| Internal bar code: | CCAAATATTTCTTGTGTACGGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 647 |
| LEAP-Seq percent confirming: | 99.6006 |
| LEAP-Seq n confirming: | 4489 |
| LEAP-Seq n nonconfirming: | 18 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAACTGCACTCGCAGCTTAG |
| Suggested primer 2: | ACGCTTATGCCTTCAGCACT |