| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.074687 |
| Chromosome: | chromosome 16 |
| Location: | 5465648 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g680900 | RPB1 | (1 of 1) K03006 - DNA-directed RNA polymerase II subunit RPB1 (RPB1, POLR2A); DNA-directed RNA polymerase II, largest subunit | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGGCGCACCTGTAAGCCGGCGACGTCGGGC |
| Internal bar code: | TTCGGCGCTCCATCTAGTGAGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1 |
| LEAP-Seq percent confirming: | 92.963 |
| LEAP-Seq n confirming: | 251 |
| LEAP-Seq n nonconfirming: | 19 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGTTGTTGCTGTGTGGAATG |
| Suggested primer 2: | GACTGCGATAAGCAACGTGA |