Insertion junction: LMJ.RY0402.074717_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.074717
Chromosome:chromosome 7
Location:4862424
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre07.g346000 (1 of 22) PTHR11017//PTHR11017:SF145 - LEUCINE-RICH REPEAT-CONTAINING PROTEIN // SUBFAMILY NOT NAMED intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ACCGCCAATGCCTGGACTACCGTGCTAACG
Internal bar code:GGCTCCGCAAATGTATCAGCTG

Confirmation - LEAP-Seq

LEAP-Seq distance:53
LEAP-Seq percent confirming:71.978
LEAP-Seq n confirming:131
LEAP-Seq n nonconfirming:51
LEAP-Seq n unique pos:3

Suggested primers for confirmation by PCR

Suggested primer 1:AGGAAATGCAGGTTACTGCG
Suggested primer 2:CAGTAGCAGCAGCAGTAGCG