Insertion junction: LMJ.RY0402.074746_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.074746
Chromosome:chromosome 17
Location:991580
Confidence (%):58
Locus systematic id Locus common name Defline Feature
Cre17.g703200 (1 of 3) IPR001071 - Cellular retinaldehyde binding/alpha-tocopherol transport CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CGCTGGCCCCGGCGCGGGCAGCTCCACCTC
Internal bar code:GGGCGGTAAGTTCGTGGGGGCC

Confirmation - LEAP-Seq

LEAP-Seq distance:64
LEAP-Seq percent confirming:95.4071
LEAP-Seq n confirming:457
LEAP-Seq n nonconfirming:22
LEAP-Seq n unique pos:3

Suggested primers for confirmation by PCR

Suggested primer 1:TTCGAAAGCTCCAAATTGCT
Suggested primer 2:GCCCTGCACCTACATGATCT