Insertion junction: LMJ.RY0402.074748_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:+
Strain:LMJ.RY0402.074748
Chromosome:chromosome 9
Location:7688468
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre09.g415550 OPR43,ASA2 Mitochondrial F1F0 ATP synthase associated protein 2 CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CGCCCTGCAAGGAGACGCACGCAGGGGATG
Internal bar code:TCGGCACTGGTAGGTACCAGAG

Confirmation - LEAP-Seq

LEAP-Seq distance:0
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:1
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:1

Suggested primers for confirmation by PCR

Suggested primer 1:AAGCACAGCAGCAACACATC
Suggested primer 2:CATTTTGGCCGTTTCCTAGA