Insertion junction: LMJ.RY0402.074759_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:+
Strain:LMJ.RY0402.074759
Chromosome:chromosome 6
Location:1022135
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre06.g256100 (1 of 19) 3.6.1.3 - Adenosinetriphosphatase / Triphosphatase 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GCATGCCATCCCCACCCTGCTCAACCCAAA
Internal bar code:CTGCTTACTCCTAATTACACGT

Confirmation - LEAP-Seq

LEAP-Seq distance:570
LEAP-Seq percent confirming:99.5971
LEAP-Seq n confirming:6922
LEAP-Seq n nonconfirming:28
LEAP-Seq n unique pos:6

Suggested primers for confirmation by PCR

Suggested primer 1:GGTTGGAACTTGGAACTGGA
Suggested primer 2:TAAATGGAGGTGTGCGATGA