| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.074785 |
| Chromosome: | chromosome 4 |
| Location: | 2977051 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre04.g224867 | (1 of 34) IPR001878 - Zinc finger, CCHC-type | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTCCGTGGCATACGCACACGCGAGCTCAGC |
| Internal bar code: | AGCTCCGAGCGAGGTCCCATTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1188 |
| LEAP-Seq percent confirming: | 99.7187 |
| LEAP-Seq n confirming: | 16306 |
| LEAP-Seq n nonconfirming: | 46 |
| LEAP-Seq n unique pos: | 12 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCAAGAAGCAGAAGACGGAC |
| Suggested primer 2: | CCAGGAACTCACTAGCCTGC |