Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | + |
Strain: | LMJ.RY0402.074824 |
Chromosome: | chromosome 6 |
Location: | 256397 |
Confidence (%): | 58 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre06.g250800 | CRB1 | (1 of 1) K13210 - far upstream element-binding protein (FUBP); C1, subunit of the circadian RNA-binding protein CHLAMY1 | 3'UTR |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGCCTCAGCAAAGCCACCCATACGCCAAGT |
Internal bar code: | CCTGGTTTTGTGACTGGTGTG |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 173 |
LEAP-Seq percent confirming: | 96.6128 |
LEAP-Seq n confirming: | 1854 |
LEAP-Seq n nonconfirming: | 65 |
LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | GTATTGCGCACAAAACCCTT |
Suggested primer 2: | ACATCGTTTTGACTCTGGGC |