| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.074832 |
| Chromosome: | chromosome 8 |
| Location: | 4331059 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre08.g381516 | (1 of 1) K11168 - dehydrogenase/reductase SDR family member 12 (DHRS12) | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTTCTCGTTCGGTCGCTCTAACTTGTTGCA |
| Internal bar code: | GAAGGAGATATCGCAGGATACT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 315 |
| LEAP-Seq percent confirming: | 99.6484 |
| LEAP-Seq n confirming: | 10203 |
| LEAP-Seq n nonconfirming: | 36 |
| LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGGTGAGAGGAGTGGGAGGT |
| Suggested primer 2: | GAGCATGGGTGCATAAGGTT |