| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.074846 |
| Chromosome: | chromosome 6 |
| Location: | 5080489 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g280600 | GEX1 | Gamete-expressed protein 1; (1 of 1) PTHR33538:SF2 - PROTEIN GAMETE EXPRESSED 1 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGGTCAGCGCTGGCGGCTCAGGCGGCGGCG |
| Internal bar code: | CAGGTCCAAATTAGGCGTGTCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 364 |
| LEAP-Seq percent confirming: | 83.3333 |
| LEAP-Seq n confirming: | 10 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCCACATCTTCCCTTCCTTG |
| Suggested primer 2: | CCACATACACAGCAACGTCC |