| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.074853 |
| Chromosome: | chromosome 13 |
| Location: | 4855068 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g605550 | (1 of 6) 2.7.7.84 - 2'-5' oligoadenylate synthase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTGAAGAAGGTATGACAGATTCTTAGTAAA |
| Internal bar code: | AACGGCTATATCTGACTCCGCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 979 |
| LEAP-Seq percent confirming: | 99.4444 |
| LEAP-Seq n confirming: | 179 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGGCGAACACTGAGAAAAAT |
| Suggested primer 2: | CCTGCCCATACCTGTGACTT |