| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.074860 |
| Chromosome: | chromosome 1 |
| Location: | 6848533 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g049400 | (1 of 3) PF04438 - HIT zinc finger (zf-HIT) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCATCGACGGGACGTCACAGGCGCGTCGGC |
| Internal bar code: | GCCAGACACTCGGTAAACCAGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 396 |
| LEAP-Seq percent confirming: | 99.2656 |
| LEAP-Seq n confirming: | 811 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 9 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTACACCAGTGAGGCCAGGT |
| Suggested primer 2: | GCGACCAGTATCAGGGATGT |