| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.074862 |
| Chromosome: | chromosome 6 |
| Location: | 2222460 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g266276 | 3'UTR_intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCGCTGCATCAGAGGCCCCAGAGGCACACT |
| Internal bar code: | CAGTTCCGAAGAACTAGGGAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1088 |
| LEAP-Seq percent confirming: | 86.6369 |
| LEAP-Seq n confirming: | 12798 |
| LEAP-Seq n nonconfirming: | 1974 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGGGGTGTTAGAATGGGTCT |
| Suggested primer 2: | TGAAGCCAAGAACTGCATTG |