| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.074863 |
| Chromosome: | chromosome 10 |
| Location: | 2341476 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g434900 | (1 of 1) PTHR11748//PTHR11748:SF35//PTHR11748:SF97 - D-LACTATE DEHYDROGENASE // D-LACTATE DEHYDROGENASE, MITOCHONDRIAL-RELATED // SUBFAMILY NOT NAMED | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AAAGACACACTACACGGCCCGCCCTTGCCT |
| Internal bar code: | TGTTCCAAGGGCGGCGAGACGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 634 |
| LEAP-Seq percent confirming: | 99.6608 |
| LEAP-Seq n confirming: | 7345 |
| LEAP-Seq n nonconfirming: | 25 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTAGGAAGGGGCAAGTAGGG |
| Suggested primer 2: | ATCGGTGAGTGCATGTGTGT |