Insertion junction: LMJ.RY0402.074900_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:+
Strain:LMJ.RY0402.074900
Chromosome:chromosome 6
Location:7788618
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre06.g302400 TNP17,CSB31 {"Probable transposon-derived protein of Chlamydomonas-Specific family B; (1 of 1) IPR000104//IPR010095 - Antifreeze protein, type I // Transposase IS605, OrfB, C-terminal 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CGCTGCGCCAGTTTCAGTGCAGCCCCCTTG
Internal bar code:TCCTAATAGCAGGTGTGTCATG

Confirmation - LEAP-Seq

LEAP-Seq distance:0
LEAP-Seq percent confirming:98.8048
LEAP-Seq n confirming:248
LEAP-Seq n nonconfirming:3
LEAP-Seq n unique pos:1

Suggested primers for confirmation by PCR

Suggested primer 1:GGTAGCTGTACGGCTGGAAG
Suggested primer 2:ACAGCCCGTGTCAGGAATAC