| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.074928 |
| Chromosome: | chromosome 3 |
| Location: | 2480790 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g159581 | 3'UTR|CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTTGTGCTGAGAGTGATCTGATTCCAGCAA |
| Internal bar code: | ACCGTAGTGACCGCGAGGTGTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 850 |
| LEAP-Seq percent confirming: | 99.4162 |
| LEAP-Seq n confirming: | 1703 |
| LEAP-Seq n nonconfirming: | 10 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGGCATGACGACTCAGCTTT |
| Suggested primer 2: | TGCATTGTTAATGCCGTTGT |