| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.074935 |
| Chromosome: | chromosome 12 |
| Location: | 2845850 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g502300 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTGGTAACCAAAGCCCAAAGGGGTTAAAGG |
| Internal bar code: | TGCTTCTGACGACGTGGGCTAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 837 |
| LEAP-Seq percent confirming: | 98.9487 |
| LEAP-Seq n confirming: | 5459 |
| LEAP-Seq n nonconfirming: | 58 |
| LEAP-Seq n unique pos: | 11 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTGGCATCTGAGCACACACT |
| Suggested primer 2: | AGTAGTAGGCCCTGCCGTTT |