| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.074938 |
| Chromosome: | chromosome 17 |
| Location: | 3356346 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g723500 | (1 of 1) 3.6.3.30 - Fe(3+)-transporting ATPase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATGGGCTTGAGCGGACCACTCGCCTTGCTG |
| Internal bar code: | CGTCTAGGCGCAGAGAGGGCTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 745 |
| LEAP-Seq percent confirming: | 99.349 |
| LEAP-Seq n confirming: | 3510 |
| LEAP-Seq n nonconfirming: | 23 |
| LEAP-Seq n unique pos: | 16 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TACTTCACACACCTGCTGCC |
| Suggested primer 2: | GCACCATGCAGTACACAACC |